h1650 luad cell lines  (Procell Inc)

Journal: Oncology Reports

Article Title: LncRNA RP11-805J14.5 functions as a ceRNA to regulate CCND2 by sponging miR-34b-3p and miR-139-5p in lung adenocarcinoma

doi: 10.3892/or.2022.8376

Figure Lengend Snippet: RP11-805J14.5 is highly expressed in LUAD. (A) The expression of RP11-805J14.5 in LUAD was defined based on the data from the TCGA dataset utilizing GEPIA. (B) The association of RP11-805J14.5 and overall survival of LUAD patients was presented based on the data from the TCGA dataset. (C) RP11-805J14.5 expression in tumor tissues and adjacent normal tissues of 60 LUAD patients was assessed using RT-qPCR. (D) The association of the expression level of RP11-805J14.5 and overall survival of 60 LUAD patients was analyzed using Kaplan-Meier analysis. (E) The expression levels of RP11-805J14.5 in human lung epithelial cell line BEAS-2B and LUAD cell lines H460, H1650, A549, H1975, and H1299 were detected using RT-qPCR. All experiments were conducted at least three times. *P<0.05, ***P<0.001 and ****P<0.0001. LUAD, lung adenocarcinoma; TCGA, The Cancer Genome Atlas; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: Human lung epithelial cell line BEAS-2B (CRL-9609), large cell lung carcinoma (LCLC) cell line H460 (HTB-177), LUAD cell lines H1650 (CRL-5883), A549 (CCL-185) and H1975 (HTB-183), and LCLC cell line H1299 (CRL-5826) were purchased from American Type Culture Collection (ATCC).

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Reports

Article Title: LncRNA RP11-805J14.5 functions as a ceRNA to regulate CCND2 by sponging miR-34b-3p and miR-139-5p in lung adenocarcinoma

doi: 10.3892/or.2022.8376

Figure Lengend Snippet: RP11-805J14.5 serves as a miR-34b-3p and miR-139-5p sponge in LUAD. (A) RT-qPCR was used to determine the levels of RP11-805J14.5 in the nucleus and cytoplasm of A549 and H1650 cells. (B) Luciferase assays were used to explore whether RP11-805J14.5 directly targeted miR-34b-3p and miR-139-5p in 293T cells. (C) RNA pull-down assays were conducted to further demonstrate the binding of RP11-805J14.5 and miR-34b-3p or miR-139-5p in A549 and H1650 cells. (D) The levels of miR-34b-3p and miR-139-5p were explored using RT-qPCR in A549 and H1650 cells with knockdown of RP11-805J14.5. (E) The levels of miR-34b-3p and miR-139-5p in tumor tissues and adjacent normal tissues of 60 LUAD patients were assessed using RT-qPCR. (F) The association of the levels of miR-34b-3p or miR-139-5p and RP11-805J14.5 was analyzed using Pearson's correlation analysis. All experiments were conducted at least three times. ***P<0.001. miR, microRNA; LUAD, lung adenocarcinoma; RT-qPCR, reverse transcription-quantitative PCR; si-, small interfering; NC, negative control; WT, wild-type; MUT, mutant.

Article Snippet: Human lung epithelial cell line BEAS-2B (CRL-9609), large cell lung carcinoma (LCLC) cell line H460 (HTB-177), LUAD cell lines H1650 (CRL-5883), A549 (CCL-185) and H1975 (HTB-183), and LCLC cell line H1299 (CRL-5826) were purchased from American Type Culture Collection (ATCC).

Techniques: Quantitative RT-PCR, Luciferase, Binding Assay, Knockdown, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control, Mutagenesis

Journal: Oncology Reports

Article Title: LncRNA RP11-805J14.5 functions as a ceRNA to regulate CCND2 by sponging miR-34b-3p and miR-139-5p in lung adenocarcinoma

doi: 10.3892/or.2022.8376

Figure Lengend Snippet: Knockdown of RP11-805J14.5 inhibits LUAD cell growth, invasion, migration and tumor growth. (A) The transfection efficiency of two siRNAs targeting RP11-805J14.5 in A549 and H1650 cells was determined using RT-qPCR. (B) Cell viability of A549 and H1650 cells with RP11-805J14.5 knockdown was determined using Cell Counting Kit-8 assay. (C) Cell apoptosis of A549 and H1650 cells with RP11-805J14.5 knockdown was determined using flow cytometry. (D) Transwell migration assays evaluated the migration abilities of A549 and H1650 cells with RP11-805J14.5 knockdown. (E) Transwell invasion assays assessed the invasion abilities of A549 and H1650 cells with RP11-805J14.5 knockdown. (F) Tumor sizes in xenograft tumor models were measured every 7 days until 28 days after establishment. (G) The expression of RP11-805J14.5 in xenograft tumors was determined using RT-qPCR. All experiments were conducted at least three times. **P<0.01 and ***P<0.001. LUAD, lung adenocarcinoma; si-, small interfering; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control.

Article Snippet: Human lung epithelial cell line BEAS-2B (CRL-9609), large cell lung carcinoma (LCLC) cell line H460 (HTB-177), LUAD cell lines H1650 (CRL-5883), A549 (CCL-185) and H1975 (HTB-183), and LCLC cell line H1299 (CRL-5826) were purchased from American Type Culture Collection (ATCC).

Techniques: Knockdown, Migration, Transfection, Quantitative RT-PCR, Cell Counting, Flow Cytometry, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control

Journal: Oncology Reports

Article Title: LncRNA RP11-805J14.5 functions as a ceRNA to regulate CCND2 by sponging miR-34b-3p and miR-139-5p in lung adenocarcinoma

doi: 10.3892/or.2022.8376

Figure Lengend Snippet: RP11-805J14.5 regulates CCND2 expression via sponging miR-34b-3p and miR-139-5p. (A) Luciferase assays were used to confirm the binding of miR-34b-3p and miR-139-5p to CCND2 in 293T cells. ***P<0.001 vs. miR-NC. (B) The mRNA levels of CCND2 were determined using RT-qPCR after the overexpression of miR-34b-3p and inhibition of miR-139-5p. ***P<0.001 vs. mimic NC or inhibitor NC. (C) The protein levels of CCND2 were determined using western blotting after the overexpression of miR-34b-3p and inhibition of miR-139-5p. (D) The mRNA levels of CCND2 were determined using RT-qPCR in A549 and H1650 cells co-transfected with siRNAs targeting RP11-805J14.5 and miR-34b-3p inhibitor or miR-139-5p inhibitor. (E) The protein levels of CCND2 were determined using western blotting in A549 and H1650 cells after co-transfection with siRNAs targeting RP11-805J14.5 and miR-34b-3p inhibitor or miR-139-5p inhibitor. All experiments were conducted at least three times. ***P<0.001 vs. si-NC; and ## P<0.01 vs. si-RP11-805J14.5. CCND2, cyclin D2; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; si-, small interfering; NC, negative control; WT, wild-type; MUT, mutant; In, inhibitor.

Article Snippet: Human lung epithelial cell line BEAS-2B (CRL-9609), large cell lung carcinoma (LCLC) cell line H460 (HTB-177), LUAD cell lines H1650 (CRL-5883), A549 (CCL-185) and H1975 (HTB-183), and LCLC cell line H1299 (CRL-5826) were purchased from American Type Culture Collection (ATCC).

Techniques: Expressing, Luciferase, Binding Assay, Quantitative RT-PCR, Over Expression, Inhibition, Western Blot, Transfection, Cotransfection, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control, Mutagenesis

Journal: Oncology Reports

Article Title: LncRNA RP11-805J14.5 functions as a ceRNA to regulate CCND2 by sponging miR-34b-3p and miR-139-5p in lung adenocarcinoma

doi: 10.3892/or.2022.8376

Figure Lengend Snippet: RP11-805J14.5 regulates LUAD progression by modulating CCND2 expression via sponging miR-34b-3p and miR-139-5p. (A) The transfection efficiency of the overexpression plasmid of CCND2 in A549 and H1650 cells was determined using reverse transcription-quantitative PCR and western blot analysis. (B) Cell viability of A549 and H1650 cells co-transfected with siRNAs targeting RP11-805J14.5 and miR-34b-3p inhibitor or miR-139-5p inhibitor or CCND2-overexpressing plasmid was determined using Cell Counting Kit-8 assays. (C) Cell apoptosis of A549 and H1650 cells co-transfected with siRNAs targeting RP11-805J14.5 and miR-34b-3p inhibitor, miR-139-5p inhibitor or CCND2-overexpressing plasmid was determined using flow cytometry. (D) Transwell migration assays determined the migration abilities of A549 and H1650 cells after co-transfection with siRNAs targeting RP11-805J14.5 and miR-34b-3p inhibitor or miR-139-5p inhibitor or CCND2-overexpressing plasmid. (E) Transwell invasion assays assessed the invasion abilities of A549 and H1650 cells after co-transfection with siRNAs targeting RP11-805J14.5 and miR-34b-3p inhibitor or miR-139-5p inhibitor or CCND2-overexpressing plasmid. All experiments were conducted at least three times. ***P<0.001 vs. si-NC; and ## P<0.01 vs. si-RP11-805J14.5. LUAD, lung adenocarcinoma; CCND2, cyclin D2; miR, microRNA; small interfering; NC, negative control; In, inhibitor.

Article Snippet: Human lung epithelial cell line BEAS-2B (CRL-9609), large cell lung carcinoma (LCLC) cell line H460 (HTB-177), LUAD cell lines H1650 (CRL-5883), A549 (CCL-185) and H1975 (HTB-183), and LCLC cell line H1299 (CRL-5826) were purchased from American Type Culture Collection (ATCC).

Techniques: Expressing, Transfection, Over Expression, Plasmid Preparation, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Cell Counting, Flow Cytometry, Migration, Cotransfection, Negative Control